On the mechanism of anaphase spindle elongation in Diatoma vulgare.

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On the mechanism of anaphase spindle elongation in Diatoma vulgare

Central spindles from five dividing cells (one metaphase, three anaphase, and one telophase) of Diatoma vulgare were reconstructed from serial sections. Each spindle is made up of two half-spindles that are composed almost entirely of polar microtubules. A small percentage of continuous microtubules and free microtubules were present in every stage except telophase. The half-spindles interdigit...

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Stu2 Promotes Mitotic Spindle Elongation in Anaphase

During anaphase, mitotic spindles elongate up to five times their metaphase length. This process, known as anaphase B, is essential for correct segregation of chromosomes. Here, we examine the control of spindle length during anaphase in the budding yeast Saccharomyces cerevisiae. We show that microtubule stabilization during anaphase requires the microtubule-associated protein Stu2. We further...

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Cross-sectional Structure of the Central Mitotic Spindle of Diatoma Vulgare

During the transition from prometaphase to metaphase, the cross-sectional area of the central spindle of Diatoma decreases by a factor of nearly two, both at the poles and at the region of overlapping microtubules (MTs) near the spindle equator . The density of spindle MT packing stays approximately constant throughout mitosis . Optical diffraction analysis of electron micrographs shows that th...

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The mechanism of anaphase spindle elongation: uncoupling of tubulin incorporation and microtubule sliding during in vitro spindle reactivation

To study tubulin polymerization and microtubule sliding during spindle elongation in vitro, we developed a method of uncoupling the two processes. When isolated diatom spindles were incubated with biotinylated tubulin (biot-tb) without ATP, biot-tb was incorporated into two regions flanking the zone of microtubule overlap, but the spindles did not elongate. After biot-tb was removed, spindle el...

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Three-dimensional structure of the central mitotic spindle of Diatoma vulgare

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1977

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.74.2.377